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The caco-2 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA)

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After that, the mixture was transferred into the column and pre-washed with wash buffer (PBS, contained 0, 10, or 30 mmol/L imidazole, pH = 7.6), followed by elution buffer (PBS, contained 300 mmol/L imidazole, pH = 7.6)

Given its critical role in BBB function and disease pathophysiology, GLUT1 remains a promising target for CNS drug delivery and therapeutic intervention [62]

10.1016/j.molcel.2010.11.010 [DOI] [PMC free article] [PubMed] [Google Scholar] Zoller H, Schaefer B, Vanclooster A et al (2022) EASL clinical practice guidelines on haemochromatosis