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The cells were split at ~7090% confluency using 0.05% trypsin-EDTA for cell detachment

10.1016/j.cell.2008.08.030 112 RojsitthisakP.JongaroonngamsangN.RomeroR

Seminotti B, Zanatta , Ribeiro RT, da Rosa MS, Wyse ATS, Leipnitz G, Wajner M

doi: 10.3390/ijerph18168697 155
Age-related hearing loss is also associated with tinnitus (ie, ringing in the ears)
