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420,421 Another limitation of CPPs is their limited clinical efficacy, often requiring high concentrations and doses to achieve a therapeutic effect, which can cause significant toxicity and side effects, hindering the development of clinical applications
Most such sensors are based on roGFP (for reduction-oxidation-sensitive GFP), which has two surface residues mutated to cysteines to allow for formation of a disulfide bond under oxidizing conditions (Lohman and Remington, 2008)
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