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To generate ground-truth data for CNN training, the FIB-SEM datasets were downsampled to isotropic 12 12 12-nm voxels, where on a fraction of NE (~1/6), locations of nuclear pores were manually annotated, and each pore was estimated as a sphere (diameter of 7 pixels)
22-363-548) - Improved Neubauer 0.1-mm-deep hemacytometer (Hausser Scientific, cat
This enables the use of powdered liposomes - free-flowing particles that immediately form a liposomal suspension when mixed with water when digested
They are water-soluble and function in the cytoplasm and mitochondria [47,76-79]
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