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FA further elevated NADPH level in thoracic aorta in Ang II-treated group and AAV-shM1 prevented this increase, indicating that FA boosts NADPH generation through MTHFD1 in mouse thoracic aorta (Fig

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In a secondary sulfur metabolic pathway, APS phosphorylation catalyzed by APS kinases (APK) forms phosphoadenosine 5-phosphosulfate (PAPS) (Takahashi et al., 2011)

293A cells were then transfected with the adenoviral expression clones using Lipofectamine 2000 (Thermo Fisher Scientific, #11668019), and crude adenoviral lysates were collected 710 days post transfection