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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Liu, M., Soden, B

doi: 10.1038/nrc704

Results vary by patient, indication, and protocol adherence

Growth hormone secretion follows a pronounced circadian pattern, with the majority of daily growth hormone release occurring during slow-wave sleep phases, particularly during the first few hours of sleep when delta wave activity is most prominent