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Similarly, substrate nicotinamide at 100 M, methyl donor S-adenosyl-L-methionine (SAM) at 5 M, and S-adenosyl methionine (SAH) at 5 M concentration) samples were also run individually in the reaction buffer [1 mM Tris [pH 8.6], 1 mM DTT, 10% trichloroacetic acid, 4% methanol, and water] to identify elution time and define substrate, co-factor, and product peaks
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Monitor for any return of symptoms and adjust accordingly
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2.4.1 Surgical procedure The rats were anesthetised with 10% ketamine HCl (50 mg/kg IM, (Alfamine) and 2% xylazine HCl (5 mg/kg IM, (Rompun)
