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2.2 Methylation specific restriction enzyme-based approaches 2.2.1 Methylation sensitive restriction enzyme (MSRE) analysis Methylation-sensitive restriction enzyme (MSRE) is initially used to selectively digest unmethylated DNA while leaving methylated targets intact

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292 This approach enables selective oriented AuNP dimerization upon target binding, greatly improving long-term stability and broadening the detection range over two orders of magnitude compared to conventional sensors

Aliquot the reconstituted peptide solution into smaller volumes to avoid thawing the entire solution multiple times

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