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Gut 62 , 11601168 (2013)

After 24 h, the fluorescent intensity of the biofilm was determined at Ex 544 nm and Em 590 nm (Tecan infinite M1000 pro)

Recent studies have increasingly shown that individuals with ASD commonly exhibit dysfunction of the autonomic nervous system, particularly characterized by parasympathetic dysregulation and reduced vagal tone (Muscatello et al., 2023

well-tolerated in mice, rats, rabbits, and dogs with no serious organ toxicity.[34][35] Clinical Safety : Mild local irritation possible at injection sites, but no systemic adverse effects reported in trials.[36][37] Potential Risks : Theoretical concerns include pro-angiogenic effects potentially promoting tumor growth, though no evidence supports this in studies.[38] Long-term human data is limited, so monitoring is advised

Code availability Bioinformatic tools, software version, parameters, and open-source code used in this present study are described in the Methods section