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Stained slides were analyzed using Qu-Path digital imagining analysis to measure staining intensity as previously described (Lee et al., 2020)

Glutathione: Proven master antioxidant for detoxification and skin brightening

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The synthesis of GSH from its constituent amino acids involves two ATP-requiring enzymatic steps: (1) the first step is rate-limiting and catalyzed by GCL which is composed of two subunits: one catalytic (GCLC) and one modifier (GCLM): (2) The second step is catalyzed by GSH synthetase (GS) [19]: Although GS is generally thought not to be important in the regulation of GSH synthesis, accumulating evidence suggests that GS may play an important role, at least in certain tissues and/or under stressful conditions [20]

Aging 26 , 251257 (2005)