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Description
We hypothesized that oxidation of GSH to glutathione disulfide (GSSG) in the epithelial lining fluid (ELF) of children with severe asthma would contribute to altered airway macrophage (AM) GSH homeostasis and AM cellular dysfunction
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The Journal of Organic Chemistry
After 12 h, cells were washed with D-PBS to remove the complete medium and loaded with 40 L of 10 M coelenterazine 400a (Maokangbio) diluted in the assay buffer (HBSS containing 0.01% bovine serum albumin and 5 mM HEPES, pH 7.5) per well
