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The beads were washed sequentially with low-salt (0.1% SDS, 1% Triton X, 2 mM EDTA, 20 mM Tris-HCl [pH 8], 150 mM NaCl), high-salt (0.1% SDS, 1% Triton X, 2 mM EDTA, 20 mM Tris-HCl [pH 8], 500 mM NaCl), LiCl (1% NP-40, 1% Sodium Deoxycholate, 1 mM EDTA, 10 mM Tris-HCl [pH 8], 250 mM LiCl), and TE (1 mM EDTA, 10 mM Tris-HCl [pH 8]) wash buffers to eliminate non-specific interactions