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Elevated Th17/Treg ratios and IL-17R-mediated NF-B activation have been documented in HT thyroid tissue (Ruggeri et al., 2014

B 13 , 1273812764 (2025)

, Ghasemlou, M

For immunofluorescence double staining with anti-FASN and anti-8-oxo-dG, paraffin sections were incubated with rabbit anti-FASN (dilution 1:50, Cell Signaling) and mouse anti-anti-8-oxo-dG (1:250, Trevigen) at 4 C o/n in a humidified chamber

Various other biomarkers of oxidative stress (for example, 8-iso-PGF2 from lipid peroxidation) were lowered concomitantly in the human study, attesting to the antioxidant functions of ERG in vivo , although in this case the healthy young subjects were probably not suffering from oxidative stress