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Cells were suspended in lysis buffer [0.1 M Tris-HCl (pH 8.0), 0.5 M NaCl] and disrupted by sonication

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Dubuquoy L, Rousseaux C, Thuru X, Peyrin-Biroulet L, Romano O, Chavatte P, Chamaillard M, Desreumaux P

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The biochemical pathway involved with the metabolism of methionine to form S-Adenosylmethionine, homocysteine and the continuation of the cycle to form other biochemicals plays a central role in the metabolism of all mammalian cells
