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The brain substantia nigra in PD has a high level of expression of the VD metabolizing enzyme and 1 hydroxylase, and VD can stimulate the expression of tyrosine hydroxylase, a key enzyme of dopamine synthesis in PD, which affects the production and turnover of dopamine, therefore, the role of VD in the development process of PD has received increasing attention (85, 86)

High doses, repeated administration, vulnerable populations (pregnant animals), and sensitive tissues (gastrointestinal tract, reproductive organs) have all been evaluated

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The human CTR was modified to contain an N-terminal Flag tag epitope and a C-terminal 8 histidine tag epitope, each flanked by a HRV 3 C protease cleavage sites

What follows reflects protocols documented in research literature and clinical practice settings