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Effector membrane translocation biosensors reveal G protein and betaarrestin coupling profiles of 100 therapeutically relevant GPCRs

Worms were transferred into glass bottles at a density of four worms per 200 ml of Hanks' Balanced Salt Solution supplemented with antibiotics (HBSS-AB: 127 mM NaCl, 7.5 mM NaHCO 3 , 5 mM KCl, 1 mM CaCl 2 , 1 mM MgCl 2 6 H 2 O), supplemented with 200 U/ml penicillin, 200 g/ml streptomycin, 2.5 g/ml amphotericin B, and 50 g/ml gentamycin (PAN-Biotech GmbH, Germany), and cultured at 37 C and 5% CO 2

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Cells 9 (8), 1806