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How Peptides Influence Sleep and Hormonal Balance Peptides that support sleep typically work through one or more of the following mechanisms: Enhancing growth hormone (GH) release during deep sleep Supporting GABAergic neurotransmission , the calming system of the brain Regulating melatonin production and circadian rhythms Reducing cortisol and stress-induced sleep disruption Improving gut-brain axis signaling , which affects serotonin and melatonin Lets explore the top peptides known to promote restful sleep and hormonal alignment