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Culture and treatment of human umbilical vein endothelial cells (HUVECs) HUVECs were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd

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People at risk of a vitamin B12 deficiency usually have conditions that hinder the absorption of B12 from their gastrointestinal system

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The biotinylation reagent sulpho-NHS-LC-biotin (Pierce, Rockford, IL) was dissolved in PBS and added to cells at 0.5 mg ml 1

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