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Gut-flora-dependent metabolite trimethylamine-N-oxide promotes atherosclerosis-associated inflammation responses by indirect ROS stimulation and signaling involving AMPK and SIRT1

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Respirometry of muscle fiber Dissected gastrocnemius muscle strips were first immersed in ice-cold isolation solution (10 mM Ca-EGTA buffer, 0.1 M free calcium, 20 mM imidazole, 20 mM taurine, 49 mM K-MES, 3 mM K 2 HPO 4 , 9.5 mM MgCl 2 , 5.7 mM ATP, 15 mM phosphocreatine, 1 M leupeptin, pH 7.1)

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Shamsi, M