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In the staining of MitoSOX Red, the cells were washed with DPBS, harvested from the flasks via trypsinization (0.05% trypsin, 0.53 mM EDTA for 2 min), washed with HBSS 2 times, stained with MitoSOX Red (5 M, Thermo Fisher Scientific Inc., USA) in HBSS at 37 C in room air for 10 min, washed with HBSS two times and diluted in HBSS for flow cytometry analysis

Oxidative stress and lung inflammation in airways disease

Evelyn Galban (Washoe and Paiute) B.S