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Bacteria were grown at 37 C to an OD 600 of ~ 0.2 and subsequently challenged with the addition of a final concentration of 20 mM of substrate: L-alanine, succinate, -ketoglutarate, L-arginine, L-histidine, L-glutamate, L-aspartate, L-glutamine and L-asparagine

Mitochondria-targeted paraquat and metformin mediate ROS production to induce multiple pathways of retrograde signaling: A dose-dependent phenomenon

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Higher doses reached that plateau later in the trial. In a 112-week step-down trial, participants maintained 21.9% loss on the top dose, 16.6% after stepping down to 5 mg, and 9.9% on placebo