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doi: 10.1093/advances/nmy023

Negative immune checkpoint regulation by VISTA: a mechanism of acquired resistance to anti-PD-1 therapy in metastatic melanoma patients

Blood was collected from the orbits every 3 days for blood tests

Vitamin C may be an important component of a comprehensive care plan, with the potential to mitigate cravings, reduce withdrawal symptoms, and address a common nutritional/metabolic etiology of illness

Positive controls should be a known protein that has already undergone purification and analysis, and that has a high-confidence set of interacting proteins identified by counterpart binding assays
