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analyzed for in-gel florescence on a ChemiDoc imaging system (Bio-Rad), stained with Coomassie or transferred to nitrocellulose membranes using a semi-dry blotter (Bio-Rad) for western blot analysis

These findings suggest that L-carnitine supplementation can potentially ameliorate chronic fatigue by attenuating oxidative stress (49)

(2015) found that transferrin makes an important impact on the induction of ferroptosis, and the importance of iron in the ferroptosis process has been further confirmed

The VAS, a numerical rating scale ranging from 0 to 100, employs a straight line without interruptions, with endpoints delineating extremes from no pain (0) to the worst imaginable pain (100)

Fixative-Based Assays Oil Red O (ORO), formerly known as Sudan Red 5B, is a fat-soluble diazol dye that specifically stains and detects neutral lipids and cholesteryl esters but not biological membranes [172]