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The plaques were manually counted using a white light transilluminator and the neutralization IC 50 that was defined as the highest antibody concentration causing a 50% plaque reduction was calculated using GraphPad Prism software
Furthermore, the expression of NGF was detected by western blotting revealed that activating the HGF/c-Met signaling pathway promotes NGF expression in the pancreatic cancer cell lines PANC-1 cells (Fig
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Science China Life Sciences

Because of lack of renal clearance, serum values rose to more than a hundredfold from 422 pmol/L on average to 54 000 pmol/L, with 67 000 pmol/L as highest value, without side-effects