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Routes used in published research Both peptides have been studied across multiple administration routes in animal models

The metabolomic analysis was performed by following the Chemical Isotope Labeling (CIL) LC-MS protocol described in our previous report (81)

Tests for POTS 10 Minute Stand Test Done in a doctors office to measure blood pressure and heart rate while standing for 10 minutes Heart rate increase of more than 30 beats per minutes with standing is diagnostic of POTS Tilt table test A more complicated test that includes continuous monitoring of heart rate and blood pressure while lying down and while suspended in an upright position on a tilt table Tests for other aspects of Dysautonomia Motility testing for gastrointestinal dysautonomia Determines what area of the gut is affected Quantitative sudomotor axon reflex test (QSART) Measures the nerves that control sweating Skin biopsy to detect small fiber neuropathy, which is commonly present in patients with dysautonomia Detects decrease in density of the small nerve fibers in the skin In the future, the Johns Hopkins Autonomic Testing Lab plans to offer : Transcranial Doppler Ultrasound Measures the change in blood flow to the brain with tilt What are the limitations of current diagnostics