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Receptor knockdown was confirmed by transfecting HEK cells with mRFP-p-actin (untransfected), a 6Myc-tagged cMet gene product that served to verify presence of protein, shRNA (c-Met) sequences (only shl was employed for the knock-down experiment) and both shRNA's combined

Iodine-based contrasts are unlikely to negatively impact the viability of platelets and I routinely advocate for its use as it often confirms proper needle placement