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Then, total RNA (2 g) was reversely transcribed into cDNA using Iscript cDNA Synthesis Kit (Bio-Rad, Milan, Italy), following the manufacturers instructions

CD47 the surface protein CD47 is a receptor that binds to signal-regulatory protein alpha (SIRP) or thrombospondin-1 (TSP-1) and acts as a signal to inactivate the phagocytosis in macrophages

This may identify the so-called parallel or non-parallel healing actions, with the final end result, positive (fistula closing) or negative (fistula remains open) (Skorjanec et al., 2009)

Recruitment of Drp1 to the OMM through interactions with Drp1 receptors, including fission protein 1 (Fis1), mitochondrial fission factor (Mff), and mitochondrial dynamics proteins (MiD49 and MiD51)

Actin is a fundamental protein that forms the cytoskeleton of cells