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5.3 Post-translational modification regulation of enzyme activity The activity of metabolic enzymes involved in the de novo serine synthesis pathway is precisely modulated by post-translational modifications such as methylation, ubiquitination and deacetylation ( Figure 3 ), and such regulatory patterns exhibit cancer-type specificity
Here are a few suggestions you can try: -Perform the IP without crosslinking the antibody to the beads -Reduce the amount of crosslinker used to covalently attach the antibody to the beads -Try a different crosslinker -To prevent co-elution of antibody, try one of our surface-activated Dynabeads magnetic beads

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