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After removal of excess dithiothreitol by gel filtration, 3Br-5MP 3e (0.02 mM, 1.4 equiv.) was added and the reaction mixture was incubated at 37 C for 1 h to generate fluorescein-modified Fab fragment 32

The most exciting prospect may be combination therapies

The approach also enables identification of the specific protein regions involved in the structural transition or affected by the binding event

platensis regulated the inflammation by suppressing the expression of the iNOS gene and protein, leading to the inhibition of NO secretion in LPS-stimulated RAW264.7 cells

Mass spectrometry studies were performed on instruments supported in part by a Center of Excellence Award by Shimadzu Inc