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Serum and gut luminal contents were analyzed in a blinded fashion for bile acids by UPLC-MS/MS at the Biomarkers Core Laboratory in the Irving Institute for Clinical and Translational Research at Columbia University Medical Center using Waters Xevo TQS mass spectrometer integrated with an Acquity UPLC system (Milford, MA), as previously described [22]

c , Targeting scheme for Slc25a39 conditional knockout (Slc25a39 fl/fl ) mice in which two loxp sites were inserted in the indicated intronic regions