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This review examines the complex mechanisms underlying MTX-induced liver damage, including oxidative stress, mitochondrial impairment, inflammatory signaling pathways such as NF-B and JAK/STAT, and apoptosis

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Histology (H&E), IHC (MUC2 and Nrf2), ROS (ELISA), gene expression (qRT-PCR), and protein levels (Western blotting) were analyzed in colon tissues

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