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Description
Cells were washed once in ice-cold PBS and lysed into RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM EDTA, 1% sodium deoxycholate, 1% Triton X-100, 0.1% sodium dodecyl sulfate [SDS]) with the addition of 1X Halt Protease and Phosphatase Inhibitor Cocktail (PI, ThermoFisher Scientific, #78440)

A: Follow the recommended dosage instructions on the product label

10.3389/fimmu.2018.01645 2 Ait-AissaK.KadlecA

Enhanced angiogenesis is a key feature of the Wolverine Stack in combined pre-clinical studies

Endocrinology 149 , 154160 (2008)
