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We concluded that the immobilized protein remained bound to the column after elution with low pH, allowing the reuse of the column for alternative uses, such as screening for other proteinprotein interactions using SPINK3 as the bait.

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It was found that addition of these alkali ions does not modify the emission wavelength of AuNCs@GSH, but fluorescence intensity undergoes a gradual increase (by ~130 %) until a plateau is reached

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