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Cycling conditions were as follows: 30 seconds at 95 C, then 40 cycles of 10 seconds at 95 C, 30 seconds at 60 C followed by a melt curve from 65 C to 95 C at 0.5 C intervals

Advances in nutrition (Bethesda, Md.) , 9 (4), 511S518S

10.1097/md.0000000000038736 170 RinninellaE.CintoniM.RaoulP.GasbarriniA.MeleM

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This produced a clear difference
