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The damage is mainly caused by superoxide anion (O 2 ), hydrogen peroxide (H 2 O 2 ), peroxide radicals (ROO ), hydroxyl radical (HO ), singlet oxygen ( 1 O 2 ) and peroxynitrite (ONOO )

[DOI] [PubMed] [Google Scholar] 89.Baum S.S., Hill R., Rommelspacher H

Multiple inputs control sulfur-containing amino acid synthesis in Saccharomyces cerevisiae

Sphingomyelin is another sphingolipid connected to autophagy

Proteins were separated by SDSPAGE, transferred to PVDF membranes (Millipore) and probed with primary antibodies in 1 TBST buffer (20 mM Tris pH 7.5, 150 mM NaCl and 0.1% Tween-20) with 5% fat-free milk at 4 C overnight