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Abbreviations ASCA anti- Saccharomyces cerevisiae antibody FGID functional gastrointestinal disorders GF germ-free IEB intestinal epithelial barrier IBD inflammatory bowel disease IBS irritable bowel syndrome IBS-C constipation-predominant irritable bowel syndrome IBS-D diarrhea-predominant irritable bowel syndrome IBS-M irritable bowel syndrome with mixed bowel habit LPS lipopolysaccharides SCFA short-chain fatty acids

Unlike traditional inorganic materials that rely predominantly on ROS generation, Cu-Mn NPs depend on Mn ions released in local tissues to enhance the antimicrobial immune response through stimulation of interferon (IFN) and pro-inflammatory cytokines via the cGAS-STING signaling pathway