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Likewise, acting through the NO-system, BPC 157 counteracted various opposite disturbances, i.e., prolonged miosis and prolonged mydriasis [111], consequences of hyperkalemia and hypokalemia [3], hypertension and hypotension [152], and bleeding and thrombosis [129,130,131,132,133,134,135,136,137,138,139,140,141,142,168]
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Viral DNA was extracted from blood samples of both captive and free-ranging khulan using a commercially available kit (QIAamp DNA Blood Mini Kit, Giagen) and from nasal swabs using the Invisorb Spin Virus DNA Mini Kit, Stratec, according to the manufactures instructions

For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, H-3300-250)
