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The gastrocnemius muscles were then excised, washed in cold phosphate-buffered saline, snap-frozen in liquid nitrogen-cooled isopentane, and stored at 80 C until analysis [2]

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When GSH reacts with DTNB, mixed disulfides (GS-S-DTNB) are formed, where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB

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