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While iPS cells are foundational for advanced organoid and humanized platforms, these constraints support the use of iPS cell-derived monocultures as complementary components within multimodel pipelines, with maximal translational value achieved through benchmarking against co-culture, ex vivo or in vivo systems that capture axonglia and immune interactions

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Furthermore, the use of 2-deoxy-D-glucose (2DG) inhibits glucokinase activity, ultimately disrupting macrophage glycolysis