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For cell culture applications, prepare fresh dilutions into serum-free media immediately before use, as serum albumin and transferrin in complete media can sequester the copper ion over extended incubation periods, altering the effective GHK-Cu concentration

doi: 10.1016/j.ijnurstu.2008.12.015 31 CuzzolinL.Francini-PesentiF.VerlatoG.JoppiM.BaldelliP.BenoniG

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J InfectDis

Collectively, these results indicate that GDF11 mitigates GC-induced vascular damage and could represent a promising therapeutic target for the treatment of GC-induced ONFH in the future