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Efficacy and safety of a drug-free, barrier-forming nasal spray for allergic rhinitis: Randomized, open-label, crossover noninferiority trial

The His 6 -tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid M pro (see Figure S1A in supplemental material) and C300S M pro were further purified by SEC using a HiLoad Superdex 200 pg column (GE Healthcare) in 20 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM DTT

However, it is widely recognized that aptamer enrichment is not always directly correlated with binding affinity due to factors such as PCR bias, selection pressure, and aptamer folding and structure 36

However, Cys112 is in range to Met94, Phe136, and Val139, which are associated to a hydrophobic pocket, important in dimerization (Vargo et al., 2004)

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