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The Journal of clinical endocrinology and metabolism 99, E2020E2024, 10.1210/jc.2014-2009 (2014)

Oxidative stress is an imbalance between free radicals (unstable molecules) and the antioxidants meant to neutralize them
The cells were harvested with a trypsin 0.05%EDTA 0.02% solution and counted in a hemocytometer

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