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Liquid chromatography-tandem mass spectrometry was employed to quantify plasma TMAO, choline and their related metabolites, encompassing TMA, l-carnitine, betaine and dimethylglycine (DMG), which are betaine-related metabolites (Reference Wiedeman, Barr and Green14)

Selected cells were maintained in DMEM plus medium (DMEM supplemented with 20% FCS, L-glutamine, 10 4 U/mL/10 4 g/mL penicillin/streptomycin, 0.8 mg/mL neomycin, and 1.0 g/mL puromycin)

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(2012) Withdrawal-associated increases and decreases in functional neural connectivity associated with altered emotional regulation in alcoholism

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