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The broodstock were injected with 10 g/kg of a Luteinizing hormone-releasing hormone (LHRH) analog after the 84-day feeding period to induce spawning

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Histopathological evaluation revealed cortical/medullary bleeding/obstruction and widespread fibrosis, dilatation, vacuolization, and degeneration in distal and proximal tubules, decreased and atypical parietal cells, and degeneration in epithelial cells

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