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A 25 to 27 gauge needle is appropriate, as it minimizes discomfort and is sufficient for penetrating the subcutaneous layer without reaching the muscle
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2.2 Parallel artificial membrane permeability assay (PAMPA) Passive membrane transport properties were measured using a 96-well pre-coated PAMPA plate system with membrane pore size 0.4 m (Gentest, Corning
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