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Description
doi: 10.1111/j.1365-2362.1995.tb01721.x [DOI] [PubMed] [Google Scholar] 123
Cost-saving by using less imidazole During purification of His-tagged proteins with conventional Ni-NTA and Ni-IDA based affinity resins, imidazole is generally required to include in the binding and wash buffers to modulate non-specific binding
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