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paragallinarum was propagated in Tryptic Soy Agar (TSA) and Tryptic Soy Broth (TSB) (Becton Dickinson, USA) enriched with 10% chicken serum and 25 g/mL NAD, and incubated at 37 C for 16 h

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The shift from maternal to embryonic regulation of development is supported by a closely controlled change in the short RNA landscape, which is reflected in this dynamic turnover [261], reduced number of hatched and unbroken blastocysts and elevated expression of Let-7a till the 8-cell stage [262], higher levels of miR-130a and miR-21 expression in zygotes compared to 8-cell stage embryos [263], In bovine embryos, elevated expression of several microRNAs, such as miR-205, miR-150, miR-96, miR-122, miR-146a, miR-145, miR-208, and miR-496, coincides with the minor and major embryonic genome activation

Research also highlights its ability to counteract drug-induced gastrointestinal side effects, offering hope to those navigating complex treatment regimens ( 2 )