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Five hundred microlitres of Dynabeads MyOne Carboxylic Acid beads (MyOne) containing 5 mg beads or 500 l of Dynabeads M-270 Carboxylic Acid beads (M-270) containing 15 mg beads were mixed with 25 nmol amino-modified HS02-52G aptamer in the presence of 500 mM EDC and incubated overnight

(2020) 64:e1901014

Any additional questions or comments from colleagues

Too much water: doses are weaker than intended If you added 3mL instead of 2mL, your concentration dropped from 2.5mg/mL to 1.67mg/mL
