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The study included 42 patients with bilateral knee osteoarthritis who were randomly assigned to receive 3 sessions of either f-ESWT or r-ESWT at 1-week intervals

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For quantitative real-time PCR (qPCR) assays, expression levels of rat 2A -adrenergic receptor ( Adra2a ) were detected using the QuantiTect SYBR Green PCR Kit (Qiagen) and SYBR Select Master Mix (Sigma-Aldrich) and quantified according to the 2 Ct method
