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What you need before starting Your peptide vial (lyophilized powder) Bacteriostatic water (USP grade, 0.9% benzyl alcohol) Sterile insulin syringes (1 mL U-100 for most applications) Alcohol swabs (70% isopropyl) Clean, flat workspace Label or marker for dating the vial Step 1: Bring everything to room temperature If your peptide vial was stored in the freezer or refrigerator, let it sit at room temperature for 15-20 minutes before reconstituting